CT115-H08H1
Human IL7RA & TSLPR Heterodimer Protein (His Tag)
Sino Biological
Image
DESCRIPTION
A DNA sequence encoding the human IL7RA (P16871-1)(Met1-Asp239) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human TSLPR (Q9HC73-1) (Met1-Lys231) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL4R & IL13RA1 heterodimer was purified.
DETAILS
- Tag: C-His
- Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
- Source: HEK293 Cells
- Species: Human
- Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: Immobilized Recombinant Human TSLP Protein (R127A, R130S, Fc Tag)(Cat:16135-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL7RA & TSLPR Heterodimer Protein (His Tag)(Cat:CT115-H08H1),the EC50 is 160-380 ng/mL.
- Molecule: IL7RA & TSLPR
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4.
- Molecular Mass: The recombinant heterodimer of human IL7RA & TSLPR comprises 450 (230+220) amino acids and has a calculated molecular mass of 52.34 (26.71+25.63) KDa. The apparent molecular mass of human IL7RA & TSLPR heterodimer is approximately 44.83 KDa respectively in SDS-PAGE.
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: P16871-1 & Q9HC73-1
- Predicted N Terminal: Glu 21 & Gln 23