• Aa: full length
• Mw: 87 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_000924
• Species: Human
• Uniprot: Q01064
• Shiptemp: -80°C (dry ice)
• Synonyms: PDE1B, phosphodiesterase 1B
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Bender A.T. and Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 103 (2), 460-465 (2006).2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005). Application Reference(s): Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013)
• Description: Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST-tag, MW= 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 380 mM NaCl, 1 mM DTT, 10% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5?-AMP per minute at 37°C.
• Assay Conditions: Assayed using BPS's PDE1B Assay Kit, Cat. # 60311. Assay conditions: 10 mM Tris-HCl,_x000D_ pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,_x000D_ 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and_x000D_ serial dilutions of PDE1B at 37°C for 20 min._x000D_ Quantified by 5'- nucleotidase cleaving the_x000D_ 5' -AMP product and releasing the_x000D_ phosphate group which is detected by_x000D_ Malachite Green Reagent.
• Specific Activity: ≥ 7600 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phosphodiesterase

DESCRIPTION