DESCRIPTION

• Notes: Troubleshooting Guide: Visit  bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email  support@bpsbioscience.com
• Species: SARS-CoV-2
• Shiptemp: -80°C (dry ice)
• Synonyms: coronavirus, covid, covid-19, covid19, Sars-cov-2 B.1.1.529, B11529, sars cov 2, sarscov2, South Africa variant, South Africa mutation assay kit, Colorimetric Spike Trimer kit, B.1.1.529 inhibitor kit, Sublineage BA.1, omicron
• Warnings: Avoid freeze/thaw cycles.
• Category: Coronavirus/Assay Kit
• Background: The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.1.529 BA.1, also known as Omicron variant, was originally discovered in South Africa and has recently become a global variant of concern. This variant contains a number of mutations that increase infectivity and transmissibility. Drugs targeting the interaction between SARS-CoV-2 Spike protein and human ACE2 may offer some protection against viral infection. This kit includes the SARS-CoV-2 Spike Trimer (B.1.1.529 BA.1, Omicron Variant) protein in its native trimeric conformation to provide a physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.
• Description: The Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2): ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the SARS-CoV-2 Omicron variant Spike Trimer and human ACE2. This kit comes in a convenient 96-well format, with Biotinylated-ACE2, purified Spike Trimer (B.1.1.529 BA.1, Omicron Variant) protein (His-tagged), Streptavidin-HRP, and assay buffers for 100 reactions. The SARS-CoV-2 Spike Trimer, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/host cell interaction. The assay requires only a few steps. First, SARS-CoV-2 Spike Trimer (B.1.1.529 BA.1, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be measured using a UV/Vis microplate reader.
• Methodology: Colorimetric
• Supplied As: This kit comes in a convenient 96-well format, with Biotinylated-ACE2, purified Spike Trimer (B.1.1.529 BA.1, Omicron Variant) protein (His-tagged), Streptavidin-HRP, and assay buffers for 100 reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: This kit is useful for screening inhibitors of ACE2 binding to SARS-CoV-2 Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant)
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 78339, 78350, 78357
• Contraindications: DMSO concentration in the final reaction should be ≤1%.
• Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
• Scientific Category: Coronavirus
• Instructions for Use: See assay protocol for detailed instructions.