DESCRIPTION

• Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
• Shiptemp: -80°C (dry ice)
• Synonyms: Immunotherapy, T cell immunoreceptor with Ig and ITIM domains, HEK293
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Cell Line
• Background: TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory T cells. Interaction with the poliovirus receptor (PVR; CD155) on antigen presenting cells, such as dendritic cells, recruits Src homology (SH) domain-containing protein tyrosine phosphatase SHP1 and SHP2 or the inositol phosphatase SHIP1 and SHIP2 to the TIGIT ITIM domain. This increases IL-10 release and suppresses NF-kB and NFAT T cell receptor (TCR) signaling, which blocks T cell proliferation and cytokine production. It serves as a competitive inhibitor of CD226, a co-stimulatory receptor for CD155. TIGIT targeting antibodies that block this T cell-intrinsic inhibitory effects have shown enhanced anti-tumor and anti-viral functions in preclinical studies.
• Description: Recombinant HEK293 stably expressing human TIGIT (T cell immunoreceptor with Ig and ITIM domains; VSTM3; VSIG9), GenBank Accession #NM_173799.
• Supplied As: Each vial contains ~3 X 10^6 cells in 1 ml of 10% DMSO.
• Unspsc Code: 41106514
• Unspsc Name: Stable mammalian cell lines
• Applications: • Screening for TIGIT binding molecules (such as anti-TIGIT antibody) in a cellular context
• Host Species: human
• Product Type: Cell Line
• Host Cell Line: HEK293
• Biosafety Level: BSL-2
• Related Products: 60538, 71218, 60187-1, 71228-2, 71340, 79269, 79540, 60187
• Storage Stability: Immediately upon receipt, store in liquid nitrogen.
• Mycoplasma Testing: The cell line has been screened using the metabolite-based Mycoplasma Detection Kit (Biotool, #B3903) to confirm the absence of Mycoplasma species.
• Scientific Category: Immunotherapy
• Instructions for Use: See cell line data sheet for detailed culturing and assay protocol.
• Required Accessories: 79540,60187,79796
• Signed License Required: Yes