DESCRIPTION

• Aa: 1 - 277 (end)
• Mw: see Description
• Tags: C-terminal His-tag
• Format: Aqueous buffer solution
• Genbank: NM_004346
• Species: Human
• Uniprot: P42574
• Shiptemp: -80°C (dry ice)
• Synonyms: caspase-3, CASP3, caspase 3, CPP32, apopain, Yama
• Warnings: Avoid freeze/thaw cycles.
• Category: Apoptosis/Protein
• Background: Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.
• References: 1. Lee, D., et al., J Biol Chem. 2000 May 26;275(21):16007-142. Suzuki, Y., et al., Proc Natl Acad Sci USA. 2001 Jul 17;98(15):866-7.
• Description: Human caspase 3 (CASP3), also known as CPP32 and apopain, a.a. 1 - 277 (end) (GenBank Accession No. NM_004346, var alpha) with C-terminal His-tag, expressed in an E. coli expression system. Procaspase-3 is self-cleaved into two subunits, MW = 17 kDa and 13 kDa, to form the active heterotetrameric complex.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assayed using BPS's Homogeneous Caspase-3 Assay Kit, Cat. # 80700. Assay conditions: 10 mM HEPES, pH 7.4, 10 mM EDTA, 0.05% Chaps, 5 mM DTT, and 20 µM Caspase 3 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em500 nm.
• Related Products: 80114-2, 70000-1, 80115, 80113-2
• Specific Activity: ≥ 6,784 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Protease