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11058-VNAHC2

Influenza A H1N1 Neuraminidase / NA (N295S mutation) (Active)

Sino Biological

DESCRIPTION

A DNA sequence encoding the Influenza A virus (A/California/04/2009 (H1N1)) neuraminidase (ACP41107.1) (Met 1-Lys 469) was expressed, the cell lysates are collected, and bio-activity was tested. There is an amino acid change from Asparagine to Serine (N295S mutation) in NA / Neuraminidase.

DETAILS

  • Tag: Native
  • Source: HEK293 Cells
  • Species: H1N1
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Measured by its ability to cleave a fluorogenic substrate, 2’-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid per minute at pH 7.5 at 37℃.
  • Molecule: NA
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, 0.6% Triton X-100, 7% Trehalose, 6% Mannitol, pH 7.4
  • Molecular Mass: The recombinant influenza A H1N1 Neuraminidase comprises 469 amino acids and has a predicted molecular mass of 52 kDa.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: ACP41107.1