RR201547L3
Fam149b1 (NM_001013878) Rat Tagged Lenti ORF Clone
Origene Technologies, Inc.
DESCRIPTION
Lenti ORF clone of Fam149b1 (Myc-DDK-tagged ORF) - Rat family with sequence similarity 149, member B1 (Fam149b1), (10 ug)
DETAILS
- Tag: Myc-DDK
- Accn: NM_001013878
- Type: Rat Tagged ORF Clone
- Refseq: NM_001013878.1, NP_001013900.1
- Status: 8 Weeks*
- Symbol: Fam149b1
- Vector: pLenti-C-Myc-DDK-P2A-Puro
- Category: cDNA Clones
- Locus Id: 289900
- Orf Size: 1446 bp
- Synonyms: RGD1359592
- Refseq Orf: 1449 bp
- Uniprot Id: Q5PQL8
- Refseq Size: 2147 bp
- Availability: 8 Weeks
- Cytogenetics: 15p16
- Gene Summary: Involved in the localization of proteins to the cilium and cilium assembly. Indirectly regulates the signaling functions of the cilium, being required for normal SHH/smoothened signaling and proper development.[UniProtKB/Swiss-Prot Function]
- Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(RR201547).
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
- E Coli Selection: Chloramphenicol (34 ug/mL)
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Puromycin