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NUN-C52Hp-100ug NUN-C52Hp-1mg

SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag

ACROBiosystems

DESCRIPTION

SARS-CoV-2 Nucleocapsid protein, His Tag (NUN-C52Hp) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1). The mutations (D63G, R203M, D377Y, R385K) were identified in the SARS-CoV-2 Delta lineages (Pango lineage: B.1.617.2; GISAID clade: 21A, 21I, 21J; Nextstrain clade: G/478K.V1).

DETAILS

  • Tag: C-6×His
  • Host: HEK293
  • Size: 100ug
  • Buffer: PBS 0.5 M Arginine,pH7.3
  • Format: Powder
  • Purity: 90%
  • Storage: -20℃
  • Category: Viral Proteins
  • Molecule: Nucleocapsid protein
  • Accession: QHO62115.1
  • Conjugate: Unconjugated
  • Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  • Background: Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Exp Region: Met 1 - Ala 419
  • Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
  • Endotoxin Level: 1.0 EU per μg
  • Molecular Weight: 47.2 kDa
  • Shipping Condition: RT
  • Species Reactivity: SARS-CoV-2
  • Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html