mouse monoclonal IgG1; TRB-3 Antibody (D-4) is an IgG1 κ mouse monoclonal TRB-3 antibody (also designated Tribbles Pseudokinase 3 antibody, TRB3 antibody, P65-Interacting Inhibitor Of NF-Kappa-B antibody, Tribbles Homolog 3 antibody, C20orf97 antibody, SKIP3 antibody, NIPK antibody, SINK antibody, Neuronal Cell Death Inducible Putative Kinase antibody, P65-Interacting Inhibitor Of NF-KappaB antibody, Chromosome 20 Open Reading Frame 97 antibody, or Tribbles Homolog 3 (Drosophila) antibody) that detects the TRB-3 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. TRB-3 Antibody (D-4) is available as both the non-conjugated anti-TRB-3 antibody form, as well as multiple conjugated forms of anti-TRB-3 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. TRB-3 (tribbles 3), also called NIPK (neuronal cell death-inducible protein kinase) disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. TRB3 binds to ATF4, inhibiting its transcriptional activation activity, and regulates activation of MAP kinases. In the liver, TRB-3 is a target for PPAR-alpha. Amounts of TRB3 RNA and protein are higher in livers of diabetic mice compared with those in wildtype mice. TRB3 contributes to insulin resistance in individuals with susceptibility to type II diabetes. Highest expression of TRB-3 is in liver, pancreas, peripheral blood leukocytes and bone marrow.