HL0-H82E5-25ug HL0-H82E5-200ug
Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTLLL) Complex Protein (Monomer, MALS verified)
ACROBiosystems
DESCRIPTION
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
DETAILS
- Tag: C-10×His & C-Avi
- Host: HEK293
- Size: 200ug
- Buffer: PBS, pH7.4
- Format: Powder
- Purity: 95%
- Source: Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTLLL) Complex Protein (HL0-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTLLL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & VMAPRTLLL).
- Storage: -20℃
- Category: MABSol® Biotin Labeled Proteins
- Molecule: HLA-E*0103 & B2M & CMV UL40 (VMAPRTLLL)
- Conjugate: Biotin-labeled
- Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
- Exp Region: Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & VMAPRTLLL peptide
- Swiss-prot: P13747 | P61769-1 &VMAPRTLLL
- Package Size: 200ug*1
- Characteristics: Biotinylated Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTLLL) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV UL40 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.2 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Endotoxin Level: 1.0 EU per μg
- Molecular Weight: 36.2 kDa and 13.8 kDa
- Shipping Condition: RT
- Species Reactivity: Human
- Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html