mouse monoclonal IgG2b; PKAα/β/γ cat Antibody (B-4) is an IgG2b κ mouse monoclonal PKA alpha / beta / gamma cat antibody (also designated PRKACA antibody, PRKACB antibody, PRKACG antibody, or protein kinase cAMP-activated catalytic subunit antibody) that detects the PKA alpha / beta / gamma cat protein of mouse, rat and human origin by WB, IP, IF and ELISA. PKAα/β/γ cat Antibody (B-4) is available as both the non-conjugated anti-PKA alpha / beta / gamma cat antibody form, as well as multiple conjugated forms of anti-PKA alpha / beta / gamma cat antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Free sample available. Limit 3 free samples for each full size Santa Cruz Biotechnology primary antibody ordered. All samples must be different. Customer responsible for shipping costs.