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50659-M08H

Mouse Inhibin beta A / INHBA / Activin A Protein (His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the pro form of mouse INHBA (NP_032406.1) (Met 1-Ser 424) was expressed, with a C-terminal polyhistidine tag.

DETAILS

  • Tag: C-His
  • Purity: > 88 % as determined by SDS-PAGE
  • Source: HEK293 Cells
  • Species: Mouse
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: 1. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His (Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:50659-M08H) is 0.161 μg/mL. 2. Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 μg/mL. 3. Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is typically 0.8-4 μg/mL.
  • Molecule: INHBA
  • Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile PBS, pH 7.4
  • Molecular Mass: The secreted recombinant mouse INHBA comprises 415 amino acids and has a calculated molecular mass of 46.5 kDa. As a result of glycosylation, the apparent molecular mass of rmINHBA is approximately 18 & 43 kDa corresponding to the mature inhibin beta A chain and the pro domain respectively in SDS-PAGE under reducing conditions.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: NP_032406.1
  • Predicted N Terminal: Ser 21