30013
TC-PTP(PTPN2), GST-Tag Recombinant
BPS Bioscience
DESCRIPTION
Human recombinant TC-PTP, also known as PTPN2 (protein tyrosine phosphatase N2), Genbank accession No. BC016727, a.a. 2-315 (catalytic domain), with N-terminal GST tag, MW=62.3 kDa, expressed in an E. coli expression system.
DETAILS
- Aa: 2-315
- Mw: 62.3 kDa
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: BC016727
- Species: Human
- Uniprot: P17706
- Shiptemp: -80°C (dry ice)
- Synonyms: PTPN2, TC-PTP, PTPT, TCELLPTP, TCPTP
- Warnings: Avoid freeze/thaw cycles.
- Category: Protein Phosphatase/Non-Receptor
- Background: Protein tyrosine phosphatase, non-receptor type 2 (PTPN2), is one of the most abundant mammalian tyrosine phosphatase. The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. By virtue of protein tyrosine phosphatase activity, PTPN2 is known to be a signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation involved in cell communication and signal transduction.
- References: 1. N.F. Zander et al., Biochemistry 1991, 30: 6964.2. D.E. Cool et al., Proc. Natl. Acad. Sci. USA 86: 5257.
- Description: Human recombinant TC-PTP, also known as PTPN2 (protein tyrosine phosphatase N2), Genbank accession No. BC016727, a.a. 2-315 (catalytic domain), with N-terminal GST tag, MW=62.3 kDa, expressed in an E. coli expression system.
- Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
- Product Type: Protein
- Biosafety Level: Not applicable (BSL-1)
- Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
- Assay Conditions: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and variable glutathione.
- Specific Activity: 463 pmole/min/µg
- Storage Stability: >12 months at -80°C
- Scientific Category: PTP/Non-rec.