DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: TCF reporter. LEF reporter, lentivirus reporter, Wnt reporter, β-catenin pathway
• Category: Cell Line/Lentiviruses
• References: 1. Tian S., et al., Blood. 1994; 84(6):1760-1764.2. Zhong, Z., et al., Science. 1994; 264(5155):95-98.
• Description: The TCF/LEF Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of TCF/LEF-responsive element located upstream of the minimal TATA promoter. After transduction, activation of the Wnt/β-catenin signaling pathway in the target cells can be monitored by measuring the luciferase activity.
• Formulation: The lentiviruses were produced from HEK293T cells and are provided in medium containing 90% DMEM + 10% FBS.
• Supplied As: Two vials (500 µl x 2) of TCF/LEF luciferase reporter lentivirus at a titer ≥1 x 10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: • Screen for activators or inhibitors of Wnt signaling pathway in the transduced target cells • Generation of TCF/LEF luciferase reporter stable cell line
• Product Type: Lentivirus
• Biosafety Level: BSL-2
• Related Products: 60500, 60501, 60690, 79665
• Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
• Scientific Category: Cell Signaling Pathway
• Instructions for Use: See assay protocol for detailed instructions.