GABA-A Receptor Alpha 4 Antibody, Clone N398A/34: RPE

DESCRIPTION:

Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1

DETAILS

Target: GABA A Receptor
Gene Id: 140675
Isotype: IgG1
Conjugate: RPE
Immunogen: Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
References: 1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602. 3. Grabenstatter H.L., et al. (2014) Epilepsia. 55(11): 1826-1833. 4. Bethmann K., Fritschy J.M., Brandt C., Loscher W. (2008) Neurbiol Dis. 31(2): 169-187.
Swiss Prot: P28471
Specificity: Detects ~60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.
Applications: WB | IHC | ICC/IF
Clone Number: N398A/34 (Formerly sold as S398A-34)
Field Of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host Species: Mouse
Product Type: Monoclonal
Purification: Protein G Purified
Concentration: 1 mg/ml
Research Areas: Neuroscience | Neurotransmitter Receptors | GABA Receptors | GABAA Receptors
Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
Accession Number: NP_542154.3
Alternative Names: GABA A receptor subunit alpha 4 Antibody, GABRA4 Antibody, Gamma aminobutyric acid A receptor Alpha 4 Antibody
Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Cite This Product: StressMarq Biosciences Cat# SMC-489D-RPE, RRID: AB_2702529
Immunogen Species: Rat
Storage Temperature: Conjugated antibodies should be stored according to the product label
Shipping Temperature: Blue Ice or 4ºC
Cellular Localization: Cell Junction | Synapse | Postsynaptic Cell Membrane | Cell Membrane
Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). GABA-A Receptor Alpha 4 is associated with epilepsy (3), and research suggests that alterations in GABA- A Receptors subtypes may be involved in the resistance to antiepileptic drugs (4).
Certificate Of Analysis: A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Species Reactivity Full Name: Human | Mouse | Rat