40450
JAK2 (JH1 domain), His-Tag Recombinant
BPS Bioscience
DESCRIPTION
Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His-tag, MW=42.5 kDa, expressed in a baculovirus expression system.
DETAILS
- Aa: 808-end
- Mw: 42.5 kDa
- Tags: N-terminal His-tag
- Format: Aqueous buffer solution
- Genbank: NM_004972
- Species: Human
- Uniprot: O60674
- Shiptemp: -80°C (dry ice)
- Synonyms: JAK2, Janus kinase-2, Just Another Kinase 2
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase/Cytoplasmic Tyrosine
- References: 1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005). 2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).
- Description: Human Jak2 (GenBank Accession No. NM_004972), (a.a. 808-end) with N-terminal His-tag, MW=42.5 kDa, expressed in a baculovirus expression system.
- Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (Tyr 4, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT._x000D_
- Related Products: 40451, 75003, 79495, 79772, 78487
- Specific Activity: 118 pmol/min/µg.
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Kinase/Cytoplasmic Tyr