DESCRIPTION

• Aa: METTL3: 2-580; METTL14: 2-456
• Mw: METTL3: 66 kDa; METTL14: 53 kDa
• Tags: METTL3: N-terminal FLAG-tag; METTL14: N-terminal His-tag
• Format: Aqueous buffer solution.
• Genbank: METTL3: NM_019852; METTL14: NM_020961
• Species: Human
• Uniprot: METTL3: Q86U44; METTL14: Q9HCE5
• Shiptemp: -80°C (dry ice)
• Synonyms: Human Methyltransferase-like 3, METTL3; Methyltransferase-like 14
• Warnings: Avoid freeze/thaw cycles. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
• Category: Methyltransferase/Enzymes
• References: 1. Liu J. et al. Nat. Chem. Biol. 2014. 10:93-95 2. Wang X. et al. Nature. 2016. 534:575-578
• Description: Complex of Human Methyltransferase-like 3 (METTL3), GenBank Accession No. NM_019852, a.a. 2-580 (end) with N-terminal FLAG-tag, MW = 66 kDa and Methyltransferase-like 14 (METTL14), Genbank Accession No. NM_020961 a.a. 2-456 (end), with N-terminal His-tag, MW = 53 kDa, coexpressed by baculovirus in Sf9 insect cells.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3mM DTT, 100ug/ml flag peptide
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Add 50µl reaction mix containing S-adenosylmethionine, METTL enzyme, and METTL buffer to wells coated with the substrate. Incubate overnight at room temperature. Add antibody against methylated adenosine, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
• Related Products: 100138, 100142, 78487
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Methyltransferase