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36-1800-100

Scientist.com Supplier

Monoclonal Antibody to Vimentin (Mesenchymal Cell Marker)(Clone : SPM576)

Abeomics

DESCRIPTION

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

DETAILS

  • Gene: VIM
  • App Id: IHC,FACS,WB
  • Antigen: Vimentin (Mesenchymal Cell Marker)
  • Gene Id: 7431
  • Isotype: Mouse IgG1, kappa
  • Format Id: Purified
  • Clone Name: SPM576
  • Uniprot Id: P08670
  • Host Animal: Mouse
  • Clonality Id: Monoclonal
  • Purification: Affinity Chromatography
  • Reactivity Id: Human
  • Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
  • Alternative Name: VIM
  • Application Note: Flow Cytometry (1-2ug/million cells); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
  • Categories Name 1: Antibodies
  • Categories Name 2: Cancer Marker
  • Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
  • Immunogen Information: Human vimentin recombinant protein