DESCRIPTION

• Category: GENE CHOICE KITS
• Keywords: 12301-250, 12300-250, QIAGEN Plasmid Mini Kit, QIAprep Spin Miniprep Kit, 12125, 27104, 27106, plasmid mini, mini kits, plasmid kits, plasmid prep, plasmid purification, endotoxin free kit, endo free, endotoxin-free, endo-free kits, Z5134
• Paragraph 1: The Gene Choice® Plasmid DNA Miniprep Kit from Genesee Scientific is a traditional alkaline-lysis method plasmid purification kit capable of isolating up to 25µg of plasmid DNA from 1-5ml bacterial cultures. Complete purification in less than 30 minutes with 3 easy steps: bind, wash, and elute. Simple and safe protocol is free of phenol and chloroform. Recovers up to 15-25µg plasmid DNA for high copy-number inputs and 0.1-5µg for low copy-number inputs. Purified plasmids have endotoxin levels lower than 50 EU/µg and are ready for downstream applications such as routine screening, DNA sequencing, cloning, transformation, etc.
• Bullet Point 1: Traditional alkaline-lysis method for purification of plasmid DNA from bacterial culture
• Bullet Point 2: Simple and safe bind, wash, elute spin column procedure free of phenol and chloroform
• Bullet Point 3: Purify up to 25µg of plasmid DNA from 1-5ml cultures in 30 minutes or less
• Bullet Point 4: Purified plasmids are ready for a variety of downstream applications including sequencing, PCR, cloning, transformation, routine screening, etc.
• Bullet Point 5: Expected yields: 15-25µg for high copy-number; 0.1-5µg for low copy-number
• Bullet Point 6: Endotoxin levels <50 EU/µg
• Manufacturer Sku: 96-308A