SEKA10259
Human CD32b / FCGR2B ELISA Pair Set
Sino Biological
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DESCRIPTION
The human CD32b / FCGR2B ELISA Pair Set is for the quantitative determinationof human CD32b / FCGR2B.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD32b / FCGR2B coated on a 96-well plate. Standards and samples are added to the wells, and any CD32b / FCGR2B present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD32b / FCGR2B monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD32b / FCGR2B present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 55 ng of recombinant CD32b. Reconstitute standard powder with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 31.25-2000
- Specificity: Human CD32b / FCGR2B
- Capture Antibody: 0.5 mg/mL of mouse anti-CD32b monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10259-MM01)
- Detection Antibody: 0.5 mg/mL rabbit anti-CD32b monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10259-R112)