DESCRIPTION

• Tag: Tag Free
• Accn: NM_001282109
• Type: Mouse Untagged Clone
• Refseq: NM_001282109.1, NP_001269038.1
• Status: 2 Weeks*
• Symbol: Slc10a7
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 76775
• Synonyms: 2410193C02Rik; P7
• Refseq Orf: 684 bp
• Insert Size: 684 bp
• Refseq Size: 3568 bp
• Availability: 2 Weeks
• Cytogenetics: 8 C1
• Gene Summary: Does not show transport activity towards bile acids or steroid sulfates (including taurocholate, cholate, chenodeoxycholate, estrone-3-sulfate, dehydroepiandrosterone sulfate (DHEAS) and pregnenolone sulfate).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (e) lacks an in-frame exon in the central coding region, compared to variant a. The encoded isoform (e) is shorter, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC226419 representing NM_001282109 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCTCTTTCTTCAGCTCTTATCAGTCACATCCATCAACGAGTGGCTTTTAAAAGGGCAGCTGCGATAT TTAATTCAGCATTTGGAAGTTTTTTGGGCATTGTTGTGACTCCGGTGCTCCTGCTGCTTTTCCTCGGTTC ATCCTCTTCGGTGCCTTTTACATCCATTTTCTCTCAGCTGTTTATGACGGTGGTGGTTCCTCTTGTCATT GGACAGATCGTCCGACGCTACATCAAGGACTGGCTGGAGAGGAAGAAGCCACCATTTGGTGTGGTCAGCA GTAGCGTGCTACTCATGATCATCTACACCACCTTCTGTGACACCTTCTCCAACCCAAACATCGACCTGGA CAAGTTCAGCCTCATCCTCATACTGTTCATAATAGTCTCCGTTCAGCTGAGCTTCATGCTTCTGACTTTC ATCTTCTCCACACGGAATAACTCGGGGTTCACACCAGCAGACACAGTGGCTATCATCTTCTGCTCCACAC ACAAGTCCCTCACCTTGGGAATCCCAATGCTGAAGATAGTGTTTGCAGGCCATGAGCATCTCTCGCTGAT ATCCGTGCCCTTGCTCATCTACCACCCAGCTCAGATTCTCCTGGGAAGTGTGTTAGTGCCAACAATAAAG TCTTGGATGGTGTCCAGGCAGAAGGGAGTGAAGCTGACAAGGCCGACAGTGTGA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin