General descriptionCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.Cells are typically stained at 1 µg/ml.Biochem/physiol ActionsCell permeable: yesPrimary TargetDNAReversible: yesPackagingPackaged under inert gasWarningToxicity: Standard Handling (A)Physical formA 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.ReconstitutionFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.Other NotesMathieu, C., et al. 1995. Exp. Cell Res.216, 13.Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.Hotz, M.A., et al. 1994. Cytometry15, 237.Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.Miller, T., et al. 1993. Biotechniques15, 1042.Gray, J.W., et al. 1982. Science238, 323.Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.Russels, W.C., et al. 1975. Nature253, 461.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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