10256-H08H
Scientist.com Supplier
Human CD64 / FCGR1A Protein (His Tag)
Sino Biological
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DESCRIPTION:
A DNA sequence encoding the extracellular domain of human CD64A (NP_000557.1) (Met 1-Pro 288) was expressed, fused with a polyhistidine tag at the C-terminus.
DETAILS
- Tag: C-His
- Purity: ≥ 95 % as determined by SDS-PAGE, ≥ 90 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS(Routinely tested).
- Source: HEK293 Cells
- Species: Human
- Storage: Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Activity: 1.Immobilized Anti-CD20(Ro) Antibody (Rituximab) at 1 μg/mL (100 μL/well) can bind Recombinant Human CD64 / FCGR1A Protein (His Tag)(Cat:10256-H08H), the EC50 0.6-2.0 ng/mL. 2.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 5.5 nM as determined in an SPR assay (Biacore T200). 3.Captured Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 13.6 nM as determined in an SPR assay (Biacore T200). 4.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 8.5 nM as determined in a BLI assay (Sartorius Octet Red384). 5.Loaded Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 4.7 nM as determined in a BLI assay (Sartorius Octet Red384). 6.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRI / CD64 recombinant protein (Cat. 10256-H08H) with an affinity constant of 0.38 nM as determined in a BLI assay (Sartorius Octet Red384). 7.Loaded Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08H) on HIS1K Biosensor, can bind Herceptin with an affinity constant of 6.36 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 8.Loaded Herceptin on proA Biosensor, can bind Recombinant Human CD64/FCGR1 Protein, His Tag (Cat. No. 10256-H08H) with an affinity constant of 0.213 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
- Category: Proteins
- Molecule: FCGR1
- Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
- Lead Time: 1-3 days
- Formulation: Lyophilized from sterile PBS, pH 7.4.
- Molecular Mass: The recombinant human CD64A consists of 284 amino acids and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant human CD64A is approximately 45-60 kDa due to glycosylation. The molecular weight of this protein is around 38-52 kDa verified by SEC-MALS (Routinely tested).
- Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Accession Number: NP_000557.1
- Predicted N Terminal: Gln 16