Anti-p130 Cas (Tyr-762), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: BCAR1
Host: Rabbit
Rrid: AB_2290493
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: p130 Cas (Tyr-762)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: BCAR1, Cas
Clonality: Rabbit Polyclonal
Immunogen: Phospho-p130 Cas (Tyr-762) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src-binding domain of rat p130 Cas. This rat sequence is the same as Tyr-664 in human p130 Cas and Tyr-668 in mouse p130 Cas. This site is also highly conserved in Cas-L (HEF1, Nedd9).
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-751) peptide before affinity purification using phospho-p130 Cas (Tyr-762) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 130
Dilution Range Wb: 1:2000
Immunogen Species: Rat
Species Reactivity: Human, Mouse, and Rat
Target Description: p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Src binding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs). Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas central substrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.
Uniprot Number Immunogen Species: Q63767
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Tyr-762