Anti-PI3 Kinase, p85 (C-terminal region) Antibody

DETAILS

Form: Protein A Purified
Gene: PIK3R1
Host: Mouse
Rrid: AB_2165546
Type: Primary Antibody
Clone: M253
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: PI3 Kinase, p85 (C-terminal region)
Isotype: IgG2a
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: PI3K
Clonality: Mouse Monoclonal
Immunogen: Clone M253 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PI3 Kinase α subunit. This region is homologous to similar regions in rat and mouse PI3 Kinase α subunit.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects an 85 kDa* band corresponding to the p85 subunit of PI3 Kinase in western blots of human A431 and Jurkat cells.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 85
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:100
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: Phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PI), PI-4-phosphate and PI-4,5-bisphosphate to catalyze the production of PI-3,4,5-triphosphate. Growth factors and hormones activate PI3K to coordinate various cellular events, such as cell growth, cell cycle entry, cell migration and cell survival. This PI3K activation is reversed by PTEN. In cancers caused by the loss of PTEN activity, PI3K is constituitively active and promotes cell proliferation. PI3Ks are composed of a catalytic subunit and a regulatory subunit. Various isoforms of the catalytic subunit (p110a, p110b and p110d) associate with regulatory subunits (p85a and p85b) with the exception of p110g which interacts with a unique p101 regulatory subunit.
Uniprot Number Immunogen Species: P27986
Post Translational Modification Type: GOmAb