DESCRIPTION

• Aa: 610-887
• Mw: 58 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Purity: ≥90%
• Genbank: NM_004438
• Species: Human
• Uniprot: P54764
• Shiptemp: -80°C (dry ice)
• Synonyms: SEK, HEK8, TYRO1
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Receptor Tyrosine
• Background: EPHA4 also known as EPH receptor A4, belongs to the ephrin receptor subfamily of protein-tyrosine kinases which have been implicated in mediating developmental events, particularly in the nervous system (1). The EPHA4 ligand ephrin-A3 is localized to the astrocytic processes that envelop the spine. Activation of EPHA4 by ephrin-A3 induces spinal retraction and reduces spine density and inhibits the interaction distorted spine shape and organization. EPHA4-null mice possess defects in the corticospinal tract and anterior commissure indicating a model in which an ephrin ligand on the axons senses EPHA4 on spinal cord cells surrounding the corticospinal tract (2).
• References: 1. Flanagan, J.G. et al: The ephrins and Eph receptors in neural development.". Annu. Rev. Neurosci. 1998;21: 309-45.2. Dottori, M. et al: EphA4 (Sek1) receptor tyrosine kinase is required for the development of the corticospinal tract. Proc. Nat. Acad. Sci. 95: 13248-13253, 1998.
• Description: Human EPHA4 (Ephrin Receptor A4), also known as TYRO1, SEK, and HEK8, GenBank Accession No. NM_004438, a.a. 610-887, with N-terminal GST-tag, MW ~58 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
• Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
• Related Products: 40194, 40192, 40191, 40190, 78487
• Specific Activity: 61 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Receptor Tyrosine