Anti-MC1 Receptor Antibody

DESCRIPTION:

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human melanocortin receptor 1. Anti-MC1 Receptor Antibody (#AMR-020) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize MC1R from human and rat samples. The antibody is unlikely to recognize MC1R from mouse samples.

DETAILS

Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Host: Rabbit
Clone: NA
Label: Unconjugated
Purity: Affinity purified on immobilized antigen.
Target: Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2
Comment: Contact Alomone Labs for technical support and product customization
Gene Id: 4157
Isotype: Rabbit IgG
Homology: Rat - 11/6 amino acids residues identical
Is Toxin: No
Sequence: (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R
Synonyms: Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2
Accession: Q01726
Clonality: Polyclonal
Lead Time: 1-2 Business Days
Reactivity: Human|Mouse
Formulation: PBS pH7.4
Applications: IC|IF|IHC|WB
Ko Validated: yes
Preservative: No Preservative
Reconstitution: 0.2 ml double distilled water (DDW).
Blocking Peptide: BLP-MR020
Negative Control: BLP-MR020
Positive Control: NA
Cited Application: IHC|ICC
Immunogen Location: 3rd intracellular loop
Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
Shipping And Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Immunogen Source Species: Human
Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
Applications May Also Work In: IC|IF|IHC|WB
Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
Product Page Scientific Background: Melanocortin receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of α-, β- and γ-melanocyte stimulating hormone (α, β, γ-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).4All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities.MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. MC1R binds its ligands with the following potency: α-MSH = ACTH > β-MSH > γ-MSH. MC1R also binds the endogenous antagonist agouti with high affinity.1, 2MC1R can be described as the "classical" melanocyte α-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between α-MSH and agouti will determine coat pigmentation as α-MSH stimulates and agouti inhibits melanin production.In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma.1,3,5 In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes.The melanocortins and particularly α-MSH have significant anti-inflammatory properties. Since α-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of α-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.6
Standard Quality Control Of Each Lot: Western blot analysis
Application Dilutions Western Blot Wb: 1:400-1:500
Antibody Concentration After Reconstitution: 1 mg/ml
Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:100