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  • References: Ingulli, E. (2007). Tracing tolerance and immunity in vivo by CFSE-labeling of administered cells. In Immunological Tolerance (pp. 365-376). Humana Press. Lyons, A. B., & Parish, C. R. (1994). Determination of lymphocyte division by flow cytometry. Journal of immunological methods, 171(1), 131-137. Parish, C. R., Glidden, M. H., Quah, B. J., & Warren, H. S. (2009). Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current protocols in immunology, 4-9.
  • Application: FC
  • Formulation: Lyophilized
  • Application Notes: CFSE has been tested by flow cytometric analysis of stimulated human peripheral blood mononuclear cells. It can be used at concentrations ranging from 0.5 to 20 uM for cell labeling. For optimal performance, it is recommended that the used concentration be empirically determined for the assay of interest.


CFSE is a reagent useful for cell tracking, proliferation studies, and cell motility studies. After passively diffusing into cells, it is converted to a fluorescent carboxyfluorescin molecule as its acetate groups are cleaved by intracellular esterases. The fluorescent CFSE is retained inside the cell for extended periods, because the molecules forms stable covalent crosslinks with the intracellular proteins. At each cell division, the intensity of the CFSE fluorescence is halved. The peak excitation of the molecule is 494 nm and the peak emission is 521 nm and it may be used with standard fixatives containing formaldehyde and permeabilization buffers that contain saponin.