UBCH5b TR-FRET Assay Kit

DESCRIPTION

UBCH5b (UBE2D2) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5b protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

DETAILS

Shiptemp: -80°C (dry ice)
Warnings: Avoid multiple freeze/thaw cycles.
Category: Ubiquitination/Assay Kit
References: 1. Saville, M.K., et al., J. Biol. Chem. 2004; 279(40): 42169-81.2. Chiang, Y.J. et al., Nature. 2000; 403: 216-220.
Description: UBCH5b (UBE2D2) is an E2 conjugating ubiquitin protein that functions in the ubiquitination of the tumor-suppressor protein p53. It is a promising drug target in cancer immunotherapy. It utilizes biotin-labeled Ubiquitin and a terbium-labeled antibody recognizing the His-tagged UBCH5b protein to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.
Methodology: TR-FRET
Supplied As: The UBCH5b TR-FRET Assay Kit is designed to measure ubiquitination activity in a homogeneous 384 reaction format.
Unspsc Code: 41121811
Unspsc Name: Laboratory microchemistry kits
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Product Type: Assay Kit
Biosafety Level: Not applicable (BSL-1)
Related Products: 80315, 80313, 79256, 79741
Storage Stability: At least 6 months from date of receipt when stored as directed.
Scientific Category: Ubiquitin
Instructions for Use: See assay protocol for detailed instructions.