40446
Scientist.com Supplier
Mouse ITK, GST-tag Recombinant
BPS Bioscience
DESCRIPTION
Murine recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_010583), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system.
DETAILS
- Aa: 352-620(end)
- Mw: 57 kDa
- Tags: N-terminal GST-tag
- Format: Aqueous buffer solution
- Genbank: NM_010583
- Species: Mouse
- Uniprot: Q03526
- Shiptemp: -80°C (dry ice)
- Synonyms: ITK, IL2-inducible T-cell kinase , Lyk, TSK
- Warnings: Avoid freeze/thaw cycles.
- Category: Kinase/Cytoplasmic Tyrosine
- Description: Murine recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_010583), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system.
- Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
- Host Species: insect
- Product Type: Protein
- Host Cell Line: Sf9 cells
- Biosafety Level: Not applicable (BSL-1)
- Assay Conditions: Z-Lyte® (Invitrogen) screening protocol and condition were used. Enzyme reaction was conducted at 30°C for 1 hours with 2 µM Tyr 01 Peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour incubation, diluted Development Reagent B was added.
- Related Products: 40447, 40445, 78429, 78487
- Specific Activity: 2.4 pmol/min/µg
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Kinase/Cytoplasmic Tyr