DESCRIPTION

• Aa: 352-620(end)
• Mw: 57 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_010583
• Species: Mouse
• Uniprot: Q03526
• Shiptemp: -80°C (dry ice)
• Synonyms: ITK, IL2-inducible T-cell kinase , Lyk, TSK
• Warnings: Avoid freeze/thaw cycles.
• Category: Kinase/Cytoplasmic Tyrosine
• Description: Murine recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_010583), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Z-Lyte® (Invitrogen) screening protocol and condition were used. Enzyme reaction was conducted at 30°C for 1 hours with 2 µM Tyr 01 Peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour incubation, diluted Development Reagent B was added.
• Related Products: 40447, 40445, 78429, 78487
• Specific Activity: 2.4 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Kinase/Cytoplasmic Tyr