SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag

DESCRIPTION

Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

DETAILS

Tag: C-6×His
Host: HEK293
Size: 1mg
Buffer: PBS, 0.2 M Arginine, pH7.4
Format: Powder
Purity: 95%
Source: SARS-CoV-2 Nucleocapsid protein (R203M, D377Y), His Tag (NUN-C52Hn) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203M, D377Y)). The mutations (R203M, D377Y) on nucleocapsid protein were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K).
Storage: -20℃
Category: Viral Proteins
Molecule: Nucleocapsid protein
Accession: QHO62115.1 (R203M, D377Y)
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Met 1 - Ala 419
Package Size: 500ug*2
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 47.3 kDa
Shipping Condition: RT
Species Reactivity: SARS-CoV-2
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