DESCRIPTION

• Tag: Tag Free
• Accn: NM_012537
• Type: Rat Untagged Clone
• Refseq: NM_012537.3, NP_036669.3
• Status: In Stock*
• Symbol: Cyp11b1
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 500892
• Synonyms: Cp11ba; RATCP11BA
• Refseq Orf: 1500 bp
• Insert Size: 1500 bp
• Refseq Size: 2691 bp
• Availability: In Stock
• Cytogenetics: 7q34
• Gene Summary: catalyzes the conversion of 11-deoxycorticosterone to corticosterone [RGD, Feb 2006]
• Sequence Data: Fully Sequenced ORF ( show hide ) >RN213770 representing NM_012537 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCTCTCAGGGTGACAGCAGATGTGTGGCTGGCAAGACCCTGGCAGTGCCTGCACAGGACGAGGGCAC TGGGCACTACGGCAAAAGTGGCCCCCAAGACACTGAAGCCCTTTGAAGCCATACCACAATACTCCAGGAA CAAGTGGCTGAAGATGATACAGATCCTGAGGGAGCAGGGCCAAGAGAACCTACACCTGGAGATGCACCAG GCCTTCCAAGAGCTGGGGCCCATTTTCAGGCACAGTGCAGGGGGAGCACAGATTGTGTCTGTGATGCTGC CTGAGGACGCTGAGAAGCTGCACCAGGTGGAGAGTATCCTCCCGCATCGGATGCCCCTGGAGCCGTGGGT GGCCCACAGGGAACTCCGTGGCCTGAGACGTGGTGTGTTCTTGCTAAATGGGGCAGACTGGCGCTTCAAC CGACTGCAGCTGAATCCAAATATGCTGTCACCAAAAGCCATTCAATCTTTTGTCCCCTTTGTGGATGTGG TAGCAAGGGACTTTGTGGAAAACCTGAAGAAGAGAATGCTGGAGAATGTTCATGGAAGCATGTCTATAAA CATTCAGTCCAATATGTTCAACTATACCATGGAAGCCAGCCATTTTGTTATTTCCGGAGAGCGTCTGGGC CTCACAGGCCATGACCTGAAACCTGAGAGCGTGACATTCACTCATGCTCTGCACTCAATGTTCAAGTCCA CCACACAGCTCATGTTCTTACCCAAGAGCTTGACTCGTTGGACAAGCACCCGGGTGTGGAAAGAACACTT TGATTCCTGGGATATCATCTCTGAGTATGTCACAAAATGTATCAAGAATGTGTATCGCGAACTGGCAGAG GGTCGCCAACAGTCCTGGAGTGTCATATCCGAGATGGTAGCACAGAGTACTCTGTCAATGGATGCCATCC ATGCCAACTCAATGGAACTTATTGCTGGAAGTGTTGACACGACAGCAATCTCCTTGGTAATGACCCTTTT TGAGCTGGCTCGGAACCCAGATGTTCAGCAGGCCCTGCGGCAGGAGAGCCTAGCAGCTGAGGCCAGCATC GTTGCTAATCCCCAGAAGGCCATGTCAGACCTGCCCTTGCTGCGGGCTGCCCTTAAAGAGACCTTGAGGC TCTACCCTGTTGGTAGCTTTGTAGAGAGAATCGTACACTCAGACCTGGTGCTTCAGAACTATCATGTCCC TGCTGGGACATTCGTCATAATTTATCTGTACTCCATGGGCCGAAACCCTGCAGTGTTCCCAAGACCTGAG CGCTATATGCCTCAGCGCTGGCTGGAGAGGAAAAGGAGTTTCCAGCATCTGGCCTTCGGCTTTGGGATGC GCCAGTGCCTGGGGCGGCGCCTGGCAGAGGTGGAGATGCTGCTCCTGCTTCACCATATGCTGAAAACCTT CCAAGTGGAGACACTGAGACAAGAGGATATGCAGATGGTTTTTCGCTTTCTTTTGATGCCCAGCTCTAGT CCTTTCCTTACTTTCCGGCCTGTGAGCTAG ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin