DESCRIPTION

• Tag: Tag Free
• Accn: NM_175933
• Type: Mouse Untagged Clone
• Refseq: NM_175933.2, NP_787947.1
• Status: 3 Weeks*
• Symbol: Pex5
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 19305
• Synonyms: AW212715; ESTM1; PTS1R; Pxr1; X83306
• Refseq Orf: 1809 bp
• Uniprot Id: O09012
• Insert Size: 1809 bp
• Refseq Size: 3074 bp
• Availability: 3 Weeks
• Cytogenetics: 6 59.15 cM
• Gene Summary: Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR and lacks an alternate in-frame exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Variants 2 and 4 encode the same protein (isoform 2).
• Sequence Data: Fully Sequenced ORF ( show hide ) >MC219516 representing NM_175933 Red =Cloning site Blue =ORF Orange =Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCAATGCGGGAGCTGGTGGAGGGCGAATGTGGGGGTGCCAACCCGCTGATGAAGCTGGCCACCCATT TCACCCAGGACAAGGCCCTTCGGCAGGAGGGACTGAGGCCTGGCCCCTGGCCTCCGGGAGCCTCAGCAGC AGAGACCGTTTCCAAGCCTTTGGGAGTAGGAACTGAAGACGAGTTGGTGTCAGAATTCCTGCAGGACCAG AATGCAACTCTTGTATCCCGAGCCCCTCAGACCTTCAAGATGGATGACCTCCTGGCAGAGATGCAGGAGA TTGAACAGTCAAACTTTCGCCAGGCTCCTCAGAGAGCCCCTGGTGTGGCAGATTTGGCCTTGTCTGAGAA TTGGGCCCAGGAGTTTCTTGCAGCTGGAGATGCTGTGGATGTAGCTCAAGATTATAATGAGACCGACTGG TCCCAAGAATTCATCGCCGAAGTTACAGACCCACTGTCCGTGTCCCCTGCCCGATGGGCTGAGGAGTATC TGGAGCAGTCTGAGGAGAAGCTGTGGTTGGGAGACCAGGAAGGATCCTCTACCGCTGATCGATGGTACGA TGAATATCATCCTGAGGAGGACCTGCAGCACACAGCCAGTGACTTTGTGTCCAAGGTGGACGACCCCAAA TTGGCTAACTCTGAGGGCACGTCAGAGGCCTGGGTTGATCAGTTTACAAGACCAGGAAACAAAATAGCTG CCCTTCAGGTGGAATTTGAACGAGCCAAGTCAGCTATAGAGTCTGATGTCGATTTCTGGGACAAGTTACA GGCAGAGTTGGAGGAGATGGCCAAGCGGGATGCCGAGGCGCACCCCTGGCTTTCTGACTATGATGACCTC ACATCCGCTTCCTATGACAAGGGTTACCAGTTTGAGGAGGAGAATCCCTTGCGTGACCACCCTCAGCCTT TTGAAGAAGGGCTGCATCGACTGGAGGAGGGAGACCTGCCCAATGCTGTGTTGCTCTTCGAGGCTGCTGT GCAGCAGGATCCTAAGCACATGGAAGCTTGGCAGTACCTGGGCACCACCCAGGCCGAGAATGAGCAAGAG CTCTTGGCTATCAGTGCCCTGCGGAGGTGTCTGGAACTAAAGCCAGATAACCGGACAGCACTGATGGCGC TGGCTGTGAGCTTTACCAATGAGTCCCTGCAGCGCCAGGCCTGTGAGACGTTGAGGGACTGGCTGCGCTA CTCACCGGCCTACGCTCATCTGGTGGCTCCTGGGGAAGAAGGGGCGACCGGGGCAGGCCCCAGCAAGCGC ATCTTGGGATCTCTGTTGTCTGACTCCCTGTTTCTTGAAGTAAAAGATCTCTTCCTGGCGGCTGTGCGTC TGGACCCTACATCCATTGACCCTGATGTGCAGTGTGGCCTGGGAGTCCTCTTCAACCTGAGCGGGGAGTA CGACAAGGCTGTGGACTGTTTCACAGCTGCCCTCAGTGTTCGCCCCAATGACTATTTGATGTGGAACAAG CTTGGGGCCACCCTGGCCAATGGGAACCAGAGTGAAGAAGCAGTGGCTGCATACCGCCGAGCCCTTGAAC TACAGCCTGGGTATATTCGGTCCCGGTATAACCTAGGCATCAGCTGCATCAACCTGGGAGCTCACCGTGA GGCTGTGGAGCACTTTCTGGAGGCTCTGAACATGCAGAGGAAGAGCCGGGGCCCTCGGGGTGAGGGGGGC GCCATGTCTGAGAACATCTGGAGTACCCTGCGCTTGGCGTTATCCATGTTAGGCCAGAGCGATGCCTATG GGGCAGCCGATGCTCGGGATCTGTCTGCCCTCCTAGCTATGTTTGGCCTGCCCCAGTGA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
• Oti Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin