Monoclonal Antibody to gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)(HMB45 + PMEL/783)

DESCRIPTION

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

DETAILS

Gene: PMEL
App Id: WB,IHC
Antigen: gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker)
Gene Id: 6490
Isotype: Mouse IgG1, kappa + Mouse IgG1, kappa
Format Id: Purified
Clone Name: HMB45 + PMEL/783
Uniprot Id: P40967
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: PMEL||D12S53E||PMEL17||SILV
Application Note: Western Blot (2-4ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes);
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: Extract of pigmented melanoma metastases from lymph nodes (HMB45); Recombinant human SILV protein (PMEL/783)