DESCRIPTION

• Aa: full length
• Mw: 67 kDa
• Tags: N-terminal His-GST-tags
• Format: Aqueous buffer solution
• Genbank: NM_016614
• Species: Human
• Uniprot: O95551
• Shiptemp: -80°C (dry ice)
• Synonyms: PDE, tyrosyl DNA phosphodiesterase2
• Warnings: Avoid freeze/thaw cycles.
• Category: Phosphodiesterase/Enzymes
• References: 1. Loughney, K, et al., Int. J. Impot. Res.2005, 17(4):320-52. Weeks, JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9
• Description: Human tyrosyl DNA phosphodiesterase 2, or TDP2, (GenBank Accession No. NM_016614), full length with N-terminal His-GST-tag, MW = 67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol and 3 mM DTT
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: Enzyme reaction was performed in a_x000D_ reaction buffer (200 µl) containing 50 mM_x000D_ Tris (pH 7.4), 1 mM MgCl2, 50 mM KCl, 1_x000D_ mM DTT, 0.1 mg/ml BSA, 2 mM T5PNP and_x000D_ 600 ng TDP2. Reaction was continuously_x000D_ monitored at 415 nm at room temperature for_x000D_ 20 min. Specific activity was calculated based on the_x000D_ molar extinction coefficient of the product (p-nitrophenol),_x000D_ e = 15,000 M-1cm-1
• Specific Activity: ≥ 186 pmol/min/µg
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Phosphodiesterase