DESCRIPTION

• Mw: 67.9 kDa
• Tag: Myc-DDK
• Accn: NM_057172
• Type: Mouse Tagged ORF Clone
• Refseq: NM_057172.4
• Status: 2 Weeks*
• Symbol: Fubp1
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 51886
• Orf Size: 1926 bp
• Synonyms: 9530027K12Rik; D3Ertd330e; FBP; Fubp; Fubp4
• Refseq Orf: 1929 bp
• Refseq Size: 6491 bp
• Availability: 2 Weeks
• Cytogenetics: 3 77.05 cM
• Gene Summary: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription (By similarity).[UniProtKB/Swiss-Prot Function]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >MR217380 representing NM_057172 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCCGACTACTCCACAGTGCCTCCGCCGTCGTCTGGCTCGGCTGGCGGCGGCGGCGGCGGAGTCGTTA ACGACGCTTTCAAAGATGCCCTGCAGAGAGCGCGGCAGATTGCAGCAAAAATTGGGGGTGATGCTGGTAC ATCATTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCCTTAGAAGATGGAGATCAGCCAGAT GCTAAGAAAGTACCTCCCCAAAATGACTCTTTTGGAGCACAGTTACCTCCAATGCATCAGCAGCAAAGAT CTGTAATGACAGAAGAATACAAAGTCCCAGATGGGATGGTCGGATTTATAATTGGCAGAGGAGGTGAACA GATCTCACGAATACAGCAGGAATCTGGATGCAAGATACAGATAGCACCTGATAGTGGTGGCCTACCAGAA AGGTCTTGTATGCTAACTGGAACACCTGAATCTGTCCAATCAGCAAAAAGATTATTGGACCAGATTGTTG AAAAGGGAAGACCAGCCCCTGGCTTTCATCATGGTGATGGACCTGGAAATGCAGTTCAGGAAATCATGAT TCCAGCCAGCAAAGCAGGACTAGTTATTGGAAAGGGGGGCGAGACTATTAAACAACTTCAGGAACGGGCT GGTGTTAAAATGGTAATGATTCAAGATGGGCCTCAAAACACTGGTGCTGATAAACCTCTTAGGATTACGG GTGACCCATACAAAGTTCAGCAAGCCAAGGAAATGGTATTAGAGTTAATTCGTGATCAAGGTGGTTTCAG AGAAGTGCGGAATGAGTATGGCTCAAGAATAGGAGGCAATGAAGGGATAGATGTCCCAATTCCAAGATTT GCTGTTGGCATTGTAATAGGAAGAAATGGAGAAATGATTAAAAAAATACAAAATGATGCTGGTGTTCGAA TTCAGTTTAAGCCAGATGATGGAACAACACCTGATAGGATAGCACAGATAACAGGACCTCCAGACAGGTG TCAGCACGCTGCAGAAATAATCACAGACCTTCTACGAAGTGTTCAGGCTGGCAATCCTGGTGGACCGGGA CCTGGTGGTCGAGGACGAGGTAGAGGTCAAGGAAACTGGAATATGGGGCCCCCGGGTGGACTCCAGGAGT TTAATTTCATTGTGCCAACTGGGAAAACTGGACTGATCATTGGAAAAGGAGGTGAAACCATAAAAAGCAT AAGCCAACAGTCTGGTGCAAGAATAGAACTGCAGAGAAGCCCTCCACCTAATGCAGATCCCAATATGAAG TTATTTACAATTCGGGGCACTCCACAGCAAATAGACTATGCTCGACAACTCATAGAAGAGAAGATTGGGG GCCCAGTAAATCCTTTAGGGCCACCTGTACCCCATGGGCCCCATGGGGTTCCAGGTCCTCATGGGCCTCC TGGACCTCCAGGGCCGGGAACTCCAATGGGACCATACAACCCTGCACCTTACAATCCAGGACCACCTGGC CCAGCTCCTCACGGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCGTATCCACATTGGCAGC AACAGGCTCCTCCTGACCCAGCCAAGGCAGGAGCAGATCCAAACTCGGCAGCTTGGGCTGCTTATTATGC TCACTATTACCAGCAGCAGGCACAACCCCCACCTGCAGCTCCTGCCGGTGCACCAGCTACAACCCAAACG AACGGACAAGGTAACTACGGAGATCAGCAGGCTCCAGCTCCAGCTGGACAGGTTGATTATACAAAGGCTT GGGAAGAATACTACAAGAAAATGGGTCAAGCAGTTCCTGCTCCTGCTGGGGCCCCACCAGGTGGTCAGCC GGATTATAGTGCAGCCTGGGCTGAGTACTATAGACAGCAAGCAGCGTATTATGCCCAGACAAGTCCCCAG GGGATGCCGCAGCATCCTCCAGCACCTCAGGGCCAA ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >MR217380 representing NM_057172 Red =Cloning site Green =Tags(s) MADYSTVPPPSSGSAGGGGGGVVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPD AKKVPPQNDSFGAQLPPMHQQQRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPE RSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNAVQEIMIPASKAGLVIGKGGETIKQLQERA GVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELIRDQGGFREVRNEYGSRIGGNEGIDVPIPRF AVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPDRIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPG PGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRSPPPNADPNMK LFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPG PAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGADPNSAAWAAYYAHYYQQQAQPPPAAPAGAPATTQT NGQGNYGDQQAPAPAGQVDYTKAWEEYYKKMGQAVPAPAGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQ GMPQHPPAPQGQ TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin