DESCRIPTION

• Mw: 34.3 kDa
• Tag: Myc-DDK
• Accn: NM_006987
• Type: Human Tagged ORF Clone
• Refseq: NM_006987.4
• Status: In Stock*
• Symbol: RPH3AL
• Vector: pCMV6-Entry
• Domains: RPH3A_effector
• Category: cDNA Clones
• Locus Id: 9501
• Orf Size: 945 bp
• Synonyms: NOC2
• Refseq Orf: 948 bp
• Uniprot Id: Q9UNE2
• Refseq Size: 2610 bp
• Availability: In Stock
• Cytogenetics: 17p13.3
• Gene Summary: The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]
• Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC223419 representing NM_006987 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCCGACACCATCTTCGGCAGCGGGAATGATCAGTGGGTTTGCCCCAATGACCGGCAGCTTGCCCTTC GAGCCAAGCTGCAGACGGGCTGGTCCGTGCACACCTACCAGACGGAGAAGCAGAGGAGGAAGCAGCACCT CAGCCCGGCGGAGGTGGAGGCCATCCTGCAGGTCATCCAGAGGGCAGAGCGGCTCGACGTCCTGGAGCAG CAGAGAATCGGGCGGCTGGTGGAGCGGCTGGAGACCATGAGGCGGAATGTGATGGGGAACGGCCTGTCCC AGTGTCTGCTCTGCGGGGAGGTGCTGGGCTTCCTGGGCAGCTCGTCGGTGTTCTGCAAAGACTGCAGGAA GAAAGTCTGCACCAAATGTGGGATCGAGGCCTCCCCTGGCCAGAAGCGGCCCCTGTGGCTGTGTAAGATC TGCAGTGAGCAAAGAGAGGTCTGGAAGAGGTCGGGGGCCTGGTTCTACAAAGGGCTCCCCAAGTATATCT TGCCCCTGAAGACCCCTGGCCGAGCTGATGACCCCCACTTCCGACCTTTGCCCACGGAACCGGCAGAGCG AGAGCCCAGAAGCTCTGAGACCAGCCGCATCTACACGTGGGCCCGAGGAAGAGTGGTTTCCAGTGACAGT GACAGTGACTCGGATCTTAGCTCCTCCAGCCTAGAGGACAGACTCCCATCCACTGGGGTCAGGGACCGGA AAGGCGACAAACCCTGGAAGGAGTCAGGTGGCAGCGTGGAGGCCCCCAGGATGGGGTTCACCCACCCGCC GGGCCACCTCTCTGGGTGCCAGAGCAGCCTGGCCAGTGGTGAGACGGGGACAGGCTCTGCTGACCCGCCA GGGGGACCCCGCCCCGGGCTGACCCGAAGGGCCCCGGTAAAAGACACACCTGGACGAGCCCCCGCTGCTG ACGCAGCTCCAGCAGGCCCCTCCAGCTGCCTGGGC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC223419 representing NM_006987 Red =Cloning site Green =Tags(s) MADTIFGSGNDQWVCPNDRQLALRAKLQTGWSVHTYQTEKQRRKQHLSPAEVEAILQVIQRAERLDVLEQ QRIGRLVERLETMRRNVMGNGLSQCLLCGEVLGFLGSSSVFCKDCRKKVCTKCGIEASPGQKRPLWLCKI CSEQREVWKRSGAWFYKGLPKYILPLKTPGRADDPHFRPLPTEPAEREPRSSETSRIYTWARGRVVSSDS DSDSDLSSSSLEDRLPSTGVRDRKGDKPWKESGGSVEAPRMGFTHPPGHLSGCQSSLASGETGTGSADPP GGPRPGLTRRAPVKDTPGRAPAADAAPAGPSSCLG TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Protein Families: Secreted Protein
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin