RC235308
CEP164 (NM_001271933) Human Tagged ORF Clone
Origene Technologies, Inc.
DESCRIPTION
CEP164 (Myc-DDK tagged) - Homo sapiens centrosomal protein 164kDa (CEP164), transcript variant 2
DETAILS
- Mw: 164 kDa
- Tag: Myc-DDK
- Accn: NM_001271933
- Type: Human Tagged ORF Clone
- Refseq: NM_001271933.1, NP_001258862.1
- Status: 8 Weeks*
- Symbol: CEP164
- Vector: pCMV6-Entry
- Category: cDNA Clones
- Locus Id: 22897
- Orf Size: 4365 bp
- Synonyms: NPHP15
- Refseq Orf: 4368 bp
- Uniprot Id: Q9UPV0
- Refseq Size: 5550 bp
- Availability: 8 Weeks
- Cytogenetics: 11q23.3
- Gene Summary: This gene encodes a centrosomal protein involved in microtubule organization, DNA damage response, and chromosome segregation. The encoded protein is required for assembly of primary cilia and localizes to mature centrioles. Defects in this gene are a cause of nephronophthisis-related ciliopathies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
- Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC235308 representing NM_001271933 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCTGGACGACCCCTCCGCATAGGAGATCAGCTGGTTCTGGAAGAAGATTATGATGAGACCTACATTC CTAGTGAGCAAGAAATTCTTGAATTTGCCCGGGAGATTGGTATTGATCCCATCAAGGAACCAGAACTGAT GTGGCTGGCGCGAGAGGGCATCGTGGCCCCACTGCCTGGAGAGTGGAAACCATGCCAGGACATCACAGGT GACATTTACTATTTCAACTTCGCCAACGGGCAGTCTATGTGGGACCATCCATGTGACGAACACTATCGGA GCTTGGTGATCCAAGAGCGGGCAAAGCTGTCAACTTCTGGGGCCATTAAGAAGAAGAAAAAAAAAAAGGA AAAGAAAGACAAGAAGGACAGAGACCCCCCCAAAAGTTCGCTGGCCTTGGGTTCCTCATTAGCCCCAGTT CATGTTCCTCTTGGGGGCCTGGCTCCTTTACGAGGTCTTGTGGATACCCCACCCTCTGCTCTTCGTGGAT CTCAAAGCGTGAGCCTGGGGAGCTCAGTGGAGTCTGGACGTCAGCTTGGAGAACTCATGCTGCCTTCACA GGGTCTCAAGACCTCTGCTTATACAAAGGGTCTCTTGGGCTCCATATATGAGGACAAGACTGCTCTCAGC CTCTTGGGTTTAGGAGAAGAAACCAATGAGGAGGATGAGGAGGAAAGTGACAACCAGAGTGTCCACAGCT CAAGTGAGCCTCTTAGGAACCTACACCTGGACATTGGGGCACTGGGGGGTGACTTTGAGTATGAGGAGTC TCTGAGAACAAGCCAGCCAGAGGAGAAGAAGGATGTTTCTCTGGATTCAGATGCTGCCGGTCCCCCTACT CCCTGCAAGCCCTCCAGCCCAGGTGCAGACAGCAGTCTGAGCAGTGCTGTTGGCAAAGGGCGACAGGGAA GTGGAGCAAGACCTGGTCTTCCAGAAAAAGAGGAAAATGAGAAGAGTGAACCTAAGATTTGCAGGAATCT GGTGACCCCCAAGGCAGACCCTACAGGCAGTGAGCCTGCCAAAGCCTCTGAAAAGGAAGCACCAGAGGAC ACAGTAGATGCAGGAGAGGAGGGTTCCAGGAGGGAAGAGGCAGCCAAGGAGCCAAAGAAGAAGGCTTCTG CTCTGGAAGAGGGCAGTTCAGACGCCAGCCAAGAACTGGAAATTAGTGAACACATGAAGGAACCACAGCT CTCAGACTCCATAGCTTCTGACCCCAAGTCCTTCCATGGCCTGGACTTCGGTTTTCGCAGCCGGATCTCG GAGCACCTGCTGGATGTTGATGTGCTTTCCCCAGTCCTGGGTGGAGCTTGTCGGCAGGCCCAGCAACCAC TGGGAATAGAAGACAAGGATGACAGCCAGTCCAGCCAAGATGAGCTGCAGAGCAAGCAGTCCAAAGGCCT GGAGGAGAGGTACCATAGGTTATCTCCTCCACTTCCACACGAGGAGCGGGCCCAGAGTCCCCCTCGCAGC CTGGCCACTGAAGAAGAGCCTCCCCAGGGCCCCGAGGGGCAGCCCGAGTGGAAGGAGGCAGAGGAGCTTG GGGAGGACTCTGCAGCCAGCCTCAGCCTGCAGCTGTCCCTCCAGAGGGAGCAGGCCCCAAGCCCACCTGC TGCCTGTGAGAAGGGCAAGGAGCAGCATTCCCAGGCCGAGGAGCTGGGCCCTGGGCAGGAAGAGGCAGAG GATCCTGAGGAGAAGGTGGCGGTCAGCCCCACCCCGCCAGTCTCTCCAGAGGTGCGATCCACAGAGCCTG TGGCTCCCCCAGAGCAGCTCTCAGAGGCTGCACTAAAGGCCATGGAAGAGGCAGTGGCCCAAGTACTCGA GCAAGACCAGAGGCACCTGCTGGAATCCAAGCAAGAGAAGATGCAGCAACTGCGGGAGAAGCTGTGCCAA GAGGAGGAAGAGGAGATCCTCCGGCTTCACCAGCAGAAAGAGCAATCTCTCAGTTCCTTGAGGGAGCGGC TGCAGAAAGCCATTGAGGAGGAGGAGGCCCGGATGAGAGAGGAGGAAAGCCAGAGGCTATCCTGGCTCCG AGCTCAGGTCCAGTCCAGCACACAAGCAGATGAGGACCAAATCAGGGCTGAGCAAGAGGCTTCCCTGCAG AAACTGAGAGAAGAGTTGGAGTCTCAACAGAAGGCTGAGAGGGCCAGCTTGGAACAGAAAAATAGGCAAA TGCTGGAGCAGCTCAAGGAAGAGATAGAGGCTTCGGAGAAGAGCGAGCAGGCTGCCCTGAATGCTGCAAA GGAGAAGGCTCTGCAGCAGCTGAGGGAGCAGCTGGAAGGGGAGAGGAAAGAAGCTGTGGCAACGCTGGAG AAGGAGCACAGTGCTGAGCTGGAGCGGCTCTGCTCCTCATTGGAGGCCAAGCACCGGGAGGTGGTCTCCA GCCTCCAGAAGAAGATACAGGAAGCTCAACAGAAAGAGGAGGCCCAGCTGCAGAAGTGCCTTGGGCAAGT GGAGCACAGAGTTCACCAGAAGTCTTATCACGTGGCTGGGTATGAGCACGAGCTCAGCAGTCTCCTGCGA GAGAAGCGCCAGGAAGTGGAAGGGGAGCATGAGAGGAGGTTGGACAAGATGAAGGAGGAGCACCAGCAAG TGATGGCTAAGGCCAGAGAGCAGTATGAAGCTGAGGAGAGGAAGCAGCGGGCTGAGCTTCTGGGGCACCT GACCGGAGAGCTGGAGCGCCTGCAGAGGGCCCATGAACGAGAACTGGAGACTGTGAGGCAGGAGCAACAC AAGCGTCTTGAGGACTTGCGGCGCCGGCACAGGGAGCAGGAAAGGAAGCTCCAGGATTTAGAGTTGGACC TTGAAACCAGAGCTAAAGATGTCAAGGCCAGATTGGCTCTGCTGGAGGTCCAGGAGGAGACCGCCCGGAG GGAGAAGCAGCAGCTGCTTGATGTGCAGAGGCAGGTTGCTCTGAAGAGTGAGGAAGCCACAGCCACCCAT CAGCAGCTGGAGGAGGCACAGAAGGAGCACACCCACCTGTTGCAGTCAAACCAGCAGCTCCGAGAAATTC TTGATGAGCTGCAGGCCCGCAAGCTGAAGCTGGAGTCCCAAGTGGATCTGCTGCAGGCTCAGAGCCAGCA ACTGCAGAAACACTTCAGCAGCCTGGAGGCTGAAGCTCAAAAGAAGCAGCACCTGTTGAGAGAAGTGACA GTTGAGGAAAATAATGCTTCCCCACATTTTGAGCCAGATCTCCATATTGAGGACCTGAGGAAATCCCTTG GAACAAACCAGACCAAAGAGGTGTCTTCTTCTCTCTCCCAGAGCAAGGAGGACTTATACTTGGACAGCCT GTCCTCCCACAATGTCTGGCACCTCCTCTCTGCTGAGGGGGTAGCCCTCCGTAGTGCCAAGGAGTTCCTT GTGCAGCAGACACGCTCCATGCGGAGGCGGCAGACAGCTCTGAAAGCTGCCCAGCAGCATTGGCGCCATG AGCTGGCCAGTGCGCAGGAGGTGGCCAAAGACCCACCAGGCATCAAGGCCCTGGAAGATATGCGCAAGAA CCTGGAGAAGGAGACCAGGCACCTGGATGAGATGAAGTCGGCCATGCGGAAAGGCCACAACCTGCTGAAG AAGAAAGAGGAGAAGCTGAATCAGTTGGAGTCCTCTCTTTGGGAAGAGGCCTCAGATGAGGGCACTCTGG GAGGATCCCCCACCAAGAAGGCAGTAACCTTCGACCTCAGTGACATGGACAGCCTGAGCAGTGAAAGTTC TGAATCTTTTTCCCCGCCTCACCTCGACTCAACCCCGAGTCTCACCTCCCGCAAGATCCACGGGCTTAGC CACTCCCTCCGGCAGATCAGCAGCCAGCTGAGCAGTGTCCTCAGCATCCTGGACAGCCTCAACCCTCAGT CGCCGCCGCCGCTCCTCGCCTCCATGCCAGCCCAGCTCCCTCCCCGGGACCCTAAGAGCACCCCCACCCC CACCTACTATGGCTCCCTGGCCAGGTTCTCAGCCTTATCATCTGCTACACCCACGTCCACCCAATGGGCC TGGGATTCAGGGCAGGGGCCCAGGCTCCCCTCCTCTGTGGCTCAAACGGTGGACGACTTCCTGTTGGAGA AGTGGCGCAAGTATTTTCCATCTGGCATCCCGCTGCTCAGCAACAGCCCCACCCCGCTGGAGAGCAGGCT GGGTTACATGTCTGCCAGTGAGCAGCTCCGGCTCCTACAGCACTCCCATTCGCAAGTCCCTGAGGCGGGC AGCACCACCTTTCAGGGCATAATTGAGGCCAACCGGAGGTGGCTGGAACGTGTCAAGAATGACCCCAGGT TACCTCTCTTCTCGTCAACACCCAAGCCAAAAGCTACTTTGAGCCTCCTGCAGCTGGGCCTTGATGAGCA CAACAGAGTGAAGGTGTATCGCTTC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC235308 representing NM_001271933 Red =Cloning site Green =Tags(s) MAGRPLRIGDQLVLEEDYDETYIPSEQEILEFAREIGIDPIKEPELMWLAREGIVAPLPGEWKPCQDITG DIYYFNFANGQSMWDHPCDEHYRSLVIQERAKLSTSGAIKKKKKKKEKKDKKDRDPPKSSLALGSSLAPV HVPLGGLAPLRGLVDTPPSALRGSQSVSLGSSVESGRQLGELMLPSQGLKTSAYTKGLLGSIYEDKTALS LLGLGEETNEEDEEESDNQSVHSSSEPLRNLHLDIGALGGDFEYEESLRTSQPEEKKDVSLDSDAAGPPT PCKPSSPGADSSLSSAVGKGRQGSGARPGLPEKEENEKSEPKICRNLVTPKADPTGSEPAKASEKEAPED TVDAGEEGSRREEAAKEPKKKASALEEGSSDASQELEISEHMKEPQLSDSIASDPKSFHGLDFGFRSRIS EHLLDVDVLSPVLGGACRQAQQPLGIEDKDDSQSSQDELQSKQSKGLEERYHRLSPPLPHEERAQSPPRS LATEEEPPQGPEGQPEWKEAEELGEDSAASLSLQLSLQREQAPSPPAACEKGKEQHSQAEELGPGQEEAE DPEEKVAVSPTPPVSPEVRSTEPVAPPEQLSEAALKAMEEAVAQVLEQDQRHLLESKQEKMQQLREKLCQ EEEEEILRLHQQKEQSLSSLRERLQKAIEEEEARMREEESQRLSWLRAQVQSSTQADEDQIRAEQEASLQ KLREELESQQKAERASLEQKNRQMLEQLKEEIEASEKSEQAALNAAKEKALQQLREQLEGERKEAVATLE KEHSAELERLCSSLEAKHREVVSSLQKKIQEAQQKEEAQLQKCLGQVEHRVHQKSYHVAGYEHELSSLLR EKRQEVEGEHERRLDKMKEEHQQVMAKAREQYEAEERKQRAELLGHLTGELERLQRAHERELETVRQEQH KRLEDLRRRHREQERKLQDLELDLETRAKDVKARLALLEVQEETARREKQQLLDVQRQVALKSEEATATH QQLEEAQKEHTHLLQSNQQLREILDELQARKLKLESQVDLLQAQSQQLQKHFSSLEAEAQKKQHLLREVT VEENNASPHFEPDLHIEDLRKSLGTNQTKEVSSSLSQSKEDLYLDSLSSHNVWHLLSAEGVALRSAKEFL VQQTRSMRRRQTALKAAQQHWRHELASAQEVAKDPPGIKALEDMRKNLEKETRHLDEMKSAMRKGHNLLK KKEEKLNQLESSLWEEASDEGTLGGSPTKKAVTFDLSDMDSLSSESSESFSPPHLDSTPSLTSRKIHGLS HSLRQISSQLSSVLSILDSLNPQSPPPLLASMPAQLPPRDPKSTPTPTYYGSLARFSALSSATPTSTQWA WDSGQGPRLPSSVAQTVDDFLLEKWRKYFPSGIPLLSNSPTPLESRLGYMSASEQLRLLQHSHSQVPEAG STTFQGIIEANRRWLERVKNDPRLPLFSSTPKPKATLSLLQLGLDEHNRVKVYRF TR TRPLEQKLISEEDLAANDILDYKDDDDK V
- Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
- Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
- Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
- E Coli Selection: Kanamycin (25 ug/mL)
- Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
- Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
- Mammalian Cell Selection: Neomycin