Phospho-PDPK1(Y9) Antibody Blocking peptide

DETAILS

Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
Gene Id: 5170
Category: Peptides; Blocking Peptides
Subtitle: Synthetic peptide
Gene Name: PDPK1
Other Names: 3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1, PDK1
Availability: 2 weeks
Bio Background: PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Bio References: Nilsen, T., et al., J. Biol. Chem. 279(6):4794-4801 (2004).Collins, B.J., et al., EMBO J. 22(16):4202-4211 (2003).Egawa, K., et al., J. Biol. Chem. 277(41):38863-38869 (2002).Sato, S., et al., J. Biol. Chem. 277(42):39360-39367 (2002).Scott, M.T., et al., EMBO J. 21(24):6771-6780 (2002).
Primary Accession: O15530
Targetspecificity: The synthetic peptide sequence used to generate the antibody AP3476a was selected from the region of human Phospho-PDPK1-Y9. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.