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40589-V08B17

SARS-CoV-2 Spike S1+S2 (T19R, L452R, E484Q, D614G, P681R, D950N, G142D) Protein (ECD, His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations T19R, L452R, E484Q, D614G, P681R, D950N, G142D) (Met1-Pro1213) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as B.1.617.3) which emerged in the India.

DETAILS

  • Tag: C-His
  • Purity: > 85 % as determined by SDS-PAGE.
  • Source: Baculovirus-Insect Cells
  • Species: SARS-CoV-2
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Molecule: Spike
  • Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0.
  • Molecular Mass: The recombinant SARS-CoV-2 Spike S1+S2 consists of 1209 amino acids and predicts a molecular mass of 134.55 kDa.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: YP_009724390.1
  • Predicted N Terminal: Val 16