Anti-IκBα (Ser-32/Ser-36), Phosphospecific Antibody

DETAILS

Form: Protein A Purified
Gene: NFKBIA
Host: Mouse
Rrid: AB_1944433
Type: Primary Antibody
Clone: 39A1413
Buffer: PBS + 0.5% BSA and 0.05% NaN3
Target: IκBα (Ser-32/Ser-36)
Isotype: IgG1
Storage: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Shipping: Blue Ice
Synonyms: IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa
Clonality: Mouse Monoclonal
Immunogen: Clone 39A1413 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 32 and 36 of human IκBα. This peptide sequence is highly conserved in mouse, rat, dog, cow, and pig IκBα.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of Jurkat cells treated with calpain inhibitor (ALLN) followed by TNFα, but the antibody does not detect this band in untreated cells.
Applications: WB, IP
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 38
Dilution Range Ip: 1:100
Dilution Range Wb: 1:500
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.
Uniprot Number Immunogen Species: P25963
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-32/Ser-36