Cas9-Expressing Jurkat Cell Line (High or Low Expression)-78136-L

DESCRIPTION

This cell line is a clonal derivative from the Cas9-Expressing Jurkat Cell Pool (BPS Bioscience, #78070). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.

DETAILS

Tags: C-terminal FLAG-tag
Notes: License Disclosure: Visit bpsbioscience.com/license for the label license and other key information about this product. Troubleshooting Guide: For all questions, please email support@bpsbioscience.com. The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.
Species: S. pyogenes
Uniprot: Q99ZW2
Shiptemp: -80°C (dry ice)
Synonyms: SpCas9 cell line, CRISPR cell line, Cas9 cell line, Jurkat human leukemia cell line
Warnings: Avoid freeze/thaw cycles.
Category: CRISPR/Cells
Background: Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Jurkat cell lines can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
Description: This cell line is a clonal derivative from the Cas9-Expressing Jurkat Cell Pool (BPS Bioscience, #78070). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.
Supplied As: Each vial contains ~2 x 10^6 cells in 1 ml of FBS with 10% DMSO.
Unspsc Code: 41106514
Unspsc Name: Stable mammalian cell lines
Applications: 1. Quickly generating knock-out cell pools or cell lines in Jurkat cells.2. Implementing sgRNA screens in Cas9-expressing Jurkat cells.
Host Species: human
Product Type: Cell Line
Host Cell Line: Jurkat
Biosafety Level: BSL-1
Related Products: 78070, 78066, 100206
Storage Stability: Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Scientific Category: CRISPR
Instructions for Use: See assay protocol for detailed instructions.
Required Accessories: 60184,78078,79796
Signed License Required: Yes