DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: Indoleamine 2,3-dioxygenase 2, IDO-2, IDO
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Assay Kit
• Background: L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 &IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
• References: 1. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.2. Seegers, N., et al. J. Biomol. Screen. 2014; 19(9): 1266-74.
• Description: The IDO2 Inhibitor Screening Assay Kit is designed to measure IDO2 enzyme inhibition. The IDO2 Inhibitor Screening Assay Kit is simple to use. Inhibitor and enzyme are added to a sample containing L-Trp substrate.
• Methodology: Colorimetric
• Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 100 reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: Useful for the study of IDO2 enzymology, screening inhibitors, and selectivity profiling.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 27337-1, 27338-1, 71194, 72021, 72023
• Contraindications: DMSO >0.5%, strong acids or bases, ionic detergents, high salt
• Storage Stability: At least 6 months from date of receipt when stored as directed.
• Scientific Category: Immunotherapy
• Instructions for Use: See assay kit datasheet for detailed protocol.