78452
AAV1 Luciferase
BPS Bioscience
DESCRIPTION
Adeno-Associated Virus Serotype 1 (AAV1) exhibits high homology with other AAV serotypes. AAV1 efficiently transduces muscle tissue, as determined by a region of the capsid protein VP1 (amino acids 350 to 430) which functions as a major determinant of tissue tropism.
These AAV particles constitutively express the firefly (Photinus pyralis) luciferase under the control of a CMV promoter.
DETAILS
- Notes: Biosafety: Recombinant AAV is inherently replication-deficient and not known to cause any human diseases. Additionally, following transduction, AAV vectors exist episomally and do not integrate into or disrupt the host cell's genome. AAV requires the use of a Biosafety Level 1 facility. BPS Bioscience recommends following all local, federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
- Species: Wild-type AAV Serotype 1
- Shiptemp: -80°C (dry ice)
- Warnings: Avoid freeze/thaw cycles.
- Category: Viral Tools/AAV
- Description: Adeno-Associated Virus Serotype 1 (AAV1) exhibits high homology with other AAV serotypes. AAV1 efficiently transduces muscle tissue, as determined by a region of the capsid protein VP1 (amino acids 350 to 430) which functions as a major determinant of tissue tropism. These AAV particles constitutively express the firefly (Photinus pyralis) luciferase under the control of a CMV promoter.
- Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.
- Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 1012 TU/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.
- Unspsc Code: 41106621
- Unspsc Name: Virus mediated expression vectors or kits
- Applications: Use as a positive control for transduction Optimize transduction assays and track protein expression over time
- Product Type: Viral Tools
- Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium 21005-1L). Purity will vary with each lot; the exact value will be provided with each shipment.
- Biosafety Level: BSL-1
- Related Products: 78443, 78461, 78470, 78453
- Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C.
- Scientific Category: AAV