The product is Anti-Taq Monoclonal Antibody, which is suitable for Hot-Start PCR. After binding with Taq DNA polymerase, it can reversibly block the activity of DNA polymerase, so as to inhibit the production of non-specific primer binding or primer dimer at ambient temperature before thermal cycling. The Anti-Taq Monoclonal Antibody has been denatured in the initial DNA denaturation of the PCR reaction, so the Hot-Start Taq polymerase can be used for subsequent PCR without Hot-Start step, which makes the operation of the Hot-Start PCR reaction simple and fast. The product is applied to the Hot-Start PCR, which can carry out specific PCR reaction, specific amplification of multi temperature cycle and low copy number target DNA under complex background, high GC content (> 60%), secondary structure and other genomic amplification and large-scale genomic amplification detection with strong background.