DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Synonyms: Isocitrate Dehydrogenase 1, IDH1, Oxalosuccinate Decarboxylase, IDHM
• Warnings: Avoid freeze/thaw cycles.
• Category: Metabolic Enzymes/Assay Kit
• Background: Isocitrate dehydrogenases are enzymes that catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been reported in a multitude of human cancers. While the wild type IDH1, produces α-ketoglutarate (α-KG) through the reduction of NADP+ to NADPH, the IDH1(R132H) mutant catalyzes conversion of α-ketoglutarate to 2-hydroxy-glutarate (2-HG) by oxidizing NADPH to NADP+. Genomic studies have identified the IDH1(R132H) mutation in various cancer types, including glioma, chondrosarcoma, and AML as well as a small percentage of prostate, lung and colon cancers.
• References: Wang, F. et al. Science 340:622-626 (2013).
• Description: The IDH1(R132H) Assay Kit is designed to measure IDH1(R132H) activity for screening and profiling applications by measuring NADPH consumption.
• Methodology: Fluorogenic
• Supplied As: The IDH1(R132H) Assay Kit comes in a convenient 96-well format, with enough purified recombinant IDH1(R132H) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Useful for screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 71075, 71099-1, 71099-2, 79309
• Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Metabolic Enzymes
• Instructions for Use: See assay kit datasheet for detailed protocol.