PP2221
Anti-Phosphotyrosine Antibody
PhosphoSolutions
DETAILS
- Form: Antigen Affinity Purified
- Gene: N/A
- Host: Rabbit
- Rrid: AB_715277
- Type: Primary Antibody
- Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
- Target: Anti-Phosphotyrosine
- Isotype: IgG
- Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
- Shipping: Blue Ice
- Synonyms: Phosphotyrosine mAb
- Clonality: Rabbit Polyclonal
- Immunogen: Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs.
- Expiration: After date of receipt, stable for at least 1 year at -20°C.
- Specificity: This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
- Applications: WB, ICC, IP
- Conjugate Exem: Unconjugated
- Physical State: Liquid
- Quality Control: Western blots performed on each lot.
- Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
- Molecular Weight: N/A
- Dilution Range Ip: 1:100
- Dilution Range Wb: 1:1000
- Immunogen Species: Many
- Dilution Range Icc: 1:100
- Species Reactivity: Chicken, Human, Mouse, and Rat
- Target Description: Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity.
- Uniprot Number Immunogen Species: N/A
- Post Translational Modification Type: Phosphorylated
- Post Translational Modification Residue: p-Tyr