Anti-iNOS (C-terminal region) Antibody

DETAILS

Form: Protein A Purified
Gene: NOS2
Host: Mouse
Rrid: AB_10704812
Type: Primary Antibody
Clone: M398
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: iNOS (C-terminal region)
Isotype: IgG2a
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: Nos2 nitric oxide synthase 2, inducible, macrophage, NOS, type II, NOSII, Hepatocyte
Clonality: Mouse Monoclonal
Immunogen: Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFNγ and LPS, or J774A.1 cells treated with LPS only.
Applications: WB, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 130
Dilution Range Wb: 1:250
Immunogen Species: Human
Dilution Range Icc: 1:50
Species Reactivity: Human, Mouse, and Rat
Target Description: Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.
Uniprot Number Immunogen Species: P35228