Anti-ATM (Ser-1981), Phosphospecific Antibody

DETAILS

Form: Protein A Purified
Gene: ATM
Host: Mouse
Rrid: AB_1944427
Type: Primary Antibody
Clone: M366
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: ATM (Ser-1981)
Isotype: IgG1
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: ataxia telangiectasia mutated, AT1 ATDC TEL1 TELO1
Clonality: Mouse Monoclonal
Immunogen: Clone M366 was generated from a phospho-peptide that included amino acids surrounding Serine 1981 in human ATM. This sequence has high homology to the conserved site in rat and mouse ATM.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody detects a 370 kDa* protein corresponding to the molecular mass of ATM on SDS-PAGE immunoblots of calyculin A treated human A431 and Jurkat Cells, but is not observed in control cells.
Applications: WB, IP, ICC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 370
Dilution Range Ip: 1:100
Dilution Range Wb: 1:1000
Immunogen Species: Human
Dilution Range Icc: 1:200
Species Reactivity: Human, Mouse, and Rat
Target Description: Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. Activation of ATM after DNA damage involves Cdk5 mediated phosphorylation of Ser-794 followed by autophosphorylation at Ser-1891. Active ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. The Cdk5–ATM pathway regulates phosphorylation and function of the ATM targets p53 and H2AX in postmitotic neurons. Other known substrates of ATM include Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1. Thus, activation of Cdk5 by DNA damage may be an important initiator of ATM-dependent regulation of cell cycle checkpoints.
Uniprot Number Immunogen Species: Q13315
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-1981