Histone H3 (Mono Methyl Lys9) Monoclonal Antibody(1E8)

DETAILS

Source: Monoclonal, Mouse
Dilution: WB: 1:1000-3000
Function: caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l
Immunogen: Synthetic Peptide of Histone H3 (Mono Methyl Lys9)
Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
Expression: Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,
Other Name: H3K9ME1; HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Reactivity: Human;Mouse;RatGenName:HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B
Formulation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Rat Gene Id: 291159/100361558
Specificity: The antibody detects endogenous Histone H3 (di methyl K9) protein.
Applications: WB
Protein Name: Histone H3.1/Histone H3.2/Histone H3.3
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Human Gene Id: 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
Mouse Gene Id: 319152/15077/15078
Observed Bandkd: 15
Molecular Weight: 15273 Da
Rat Swiss Prot No: Q6LED0/P84245
Storage Stability: -20°C/1 year
Human Swiss Prot No: P68431/Q71DI3/P84243
Subcellular Location: Nucleus. Chromosome.